Abstract:The effects of basic medium, plant hormones, activated carbon and support material on rooting were researched in this experiment, in which Semeu Trigonellae plantlets were used as materials. The results showed that the best basic medium for rooting of Semeu Trigonellae plantlets was WPM. When WPM was used as medium and added with several plant hormones of different concentrations, the optimal result is when the plant hormone was IBA0. 25mg/ L, with the rooting rate, average root number and average root length reaching up to40. 0%, 2.3 and1.8 cm, respectively. When WPM+ IBA0. 25 mg/ L was added with activated carbon2. 0 mg/ L, the rooting efficiency was much better than that of condition when no activated carbon was added to medium, with the rooting rate, average root number and average root length reaching up to80. 0%, 4. 2 and3. 5 cm, respectively. Similarly when the WPM+ IBA0. 25 mg/ L was added with support6. 8 mg/ L carrageenan and6. 8 mg/ L agar respectively, it was found that the better choice was the medium added with carrageenan, the rooting rate reached up to75%, the average root number and average root length reached to5. 4 and3. 0 cm. Based on the aboveexperiments, the technological data could be obtained to establish Semeu Trigonellae propagation system.