Abstract: To get to know about the overall picture concerning the anti-frustration ability of the Han, Tibetan and Yi middle and high school students as well as their differences in terms of the factors like their ethnic group, region, school and family, a self-prepared“Anti-frustration ability questionnaire for middle and high school students” was adopted to give a holis- tic test to more than1002 students from different regions through the methods of T-test and variance analysis. The results showed that:(1) In general, the Han, Tibetan and Yi students had a rather good anti-frustration ability, of which the aware- ness of frustration being strongest and yet the willpower against frustration weakest. (2) The anti-frustration ability varied markedly in terms of ethnic groups, regions(cities and countries) , schools, left-behind experiences and family economic condi- tions. (3) Regression analysis showed that ethnic group, whether or not a left-behind student and family economic conditions had significant predictive effects on students’ anti-frustration ability. Factors like the ethnic group, grade, stay-at-home expe- rience and family economic level could all have influence on students’ anti-frustration ability.